Analysis and discrimination of ten different sponges by multi-step infrared spectroscopy

作者：Gan, Jian-Hong; Xu, Chang-Hua; Zhu, Hong-Zhe; Mao, Fang; Yang, Fan^*; Zhou, Qun; Sun, Su-Qin

来源：Chinese Chemical Letters, 2015, 26(2): 215-220.

DOI：10.1016/j.cclet.2015.01.012

• Sponge
• Discrimination
• Infrared spectroscopy
• Second derivative infrared spectroscopy
• Two-dimensional correlation infrared spectroscopy

In this study, a convenient method using multi-step infrared spectroscopy, including Fourier transform infrared spectroscopy (FT-IR), second derivative infrared spectroscopy (SD-IR) and two-dimensional correlation infrared spectroscopy (2DCOS-IR), was employed to analyze and discriminate ten marine sponges from two classes collected from the Xisha Islands in the South China Sea. Each sponge had an exclusive macroscopic fingerprint. From the IR spectra, it was noted that the main ingredient of calcareous sponges was calcium carbonate, but that of demosponges was proteins. For sponges from the same genus or having highly similar chemical profile (IR spectral profile), SD-IR and 2DCOS-IR were applied to successfully reveal the tiny differences. It was demonstrated that the multi-step infrared spectroscopy was a feasible and objective approach for marine sponge identification.

• 出版日期2015-2
• 单位上海交通大学; 清华大学; 上海海洋大学; 中国人民解放军第二军医大学; 天津市中医药研究院附属医院