A comparison between radiometric and fluorimetric methods for measuring SSAO activity

作者:Gella Alejandro; Sole Montse; Bolea Irene; Ventriglia Mariacarla; Siotto Mariacristina; Durany Nuria; Squitti Rosanna; Unzeta Mercedes*
来源:Journal of Neural Transmission, 2013, 120(6): 1015-1018.
DOI:10.1007/s00702-013-0987-z

摘要

Semicarbazide-sensitive amine oxidase (SSAO) metabolizes the oxidative deamination of primary aromatic and aliphatic amines. The final cytotoxic products of its catalysis contribute to diseases involving vascular degeneration. The increasing interest in measuring SSAO activity has led to the development of several different methods. Herein, we compare SSAO activity results obtained with radiometric and fluorimetric methods in 49 plasma samples. Although not interchangeable, a significant correlation was obtained between methods. Considering these limitations, the fluorimetric method might replace the radioisotopic one.

  • 出版日期2013-6
  • 单位中国人民解放军空军预警学院