Development of a Rapid and Accurate Identification Method for Citrobacter Species Isolated from Pork Products Using a Matrix-Assisted Laser-Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS)

作者：Kwak Hye Lim; Han Sun Kyung; Park Sunghoon; Park Si Hong; Shim Jae Yong; Oh Mihwa; Ricke Steven C; Kim Hae Yeong^*

来源：Journal of Microbiology and Biotechnology, 2015, 25(9): 1537-1541.

DOI：10.4014/jmb.1503.03071

• Citrobacter species
• MALDI-TOF MS
• pork products
• identification

Previous detection methods for Citrobacter are considered time consuming and laborious. In this study, we have developed a rapid and accurate detection method for Citrobacter species in pork products, using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). A total of 35 Citrobacter strains were isolated from 30 pork products and identified by both MALDI-TOF MS and 16S rRNA gene sequencing approaches. All isolates were identified to the species level by the MALDI-TOF MS, while 16S rRNA gene sequencing results could not discriminate them clearly. These results confirmed that MALDI-TOF MS is a more accurate and rapid detection method for the identification of Citrobacter species.

• 出版日期2015-9