A sensitive liquid chromatography coupled with mass spectrometry method for the intracellular and plasma quantification of raltegravir after solid-phase extraction

作者：Mosnier Thoumas Stephanie^*; Djabarouti Sarah; Xuereb Fabien; Lazaro Estibaliz; Pellegrin Jean Luc; Saux Marie Claude; Breilh Dominique

来源：Journal of Pharmacy and Pharmacology, 2011, 63(12): 1559-1565.

DOI：10.1111/j.2042-7158.2011.01368.x

• cellular concentration
• HPLC-MS
• raltegravir
• solid phase extraction

Introduction Liquid chromatography coupled with mass spectrometry for the quantification of raltegravir in human plasma and peripheral blood mononuclear cells has been developed.
Methods Sample preparations were based on a fully automated solid-phase extraction process. Mass spectrometric data were acquired in a single-ion monitoring method. Raltegravir and quinoxaline, the internal standard, were well separated in a gradient mode over 15 min.
Key findings Validation study exhibited excellent linearity, with good intra- and inter-day precision and accuracy.
Conclusions The assay was successfully applied to the raltegravir quantification in HIV-infected patients.

• 出版日期2011-12
• 单位河南工业大学