Development and Clinical Evaluation of a Rapid Serodiagnostic Test for Toxoplasmosis of Cats Using Recombinant SAG1 Antigen

作者：Chong Chom Kyu; Jeong Wooseog; Kim Hak Yong; An Dong Jun; Jeoung Hye Young; Ryu Jeong Eun; Ko A Ra; Kim Yong Joo; Hong Sung Jong; Yang Zhaoshou; Nam Ho Woo^*

来源：Korean Journal of Parasitology, 2011, 49(3): 207-212.

DOI：10.3347/kjp.2011.49.3.207

• Toxoplasma gondii
• cat
• rapid diagnostic test
• serodiagnosis
• recombinant SAG1

Rapid serodiagnostic methods for Toxoplasma gondii infection in cats are urgently needed for effective control of transmission routes toward human infections. In this work, 4 recombinant T gondii antigens (SAG1, SAG2, GRA3, and GRA6) were produced and tested for the development of rapid diagnostic test (RDT). The proteins were expressed in Escherichia coli, affinity-purified, and applied onto the nitrocellulose membrane of the test strip. The recombinant SAG1 (rSAG1) showed the strongest antigenic activity and highest specificity among them. We also performed clinical evaluation of the rSAG1-loaded RDT in 182 cat sera (55 household and 127 stray cats). The kit showed 0.88 of kappa value comparing with a commercialized ELISA kit, which indicated a significant correlation between rSAG1-loaded RDT and the ELISA kit. The overall sensitivity and specificity of the ROT were 100% (23/23) and 99.4% (158/159), respectively. The rSAG1-loaded ROT is rapid, easy to use, and highly accurate. Thus, it would be a suitable diagnostic tool for rapid detection of antibodies in T gondii-infected cats under field conditions.

• 出版日期2011-9