Nano Endoscopy with Plasmon-Enhanced Fluorescence for Sensitive Sensing Inside Ultrasmall Volume Samples

作者：Yuan, Hang; Liu, Jie; Lu, Yuexiang^*; Wang, Zhe; Wei, Guoyu; Wu, Tianhao; Ye, Gang; Chen, Jing^*; Zhang, Sichun; Zhang, Xinrong

来源：Analytical Chemistry, 2017, 89(2): 1045-1048.

DOI：10.1021/acs.analchem.6b03876

• REAL-TIME QUANTIFICATION
• POLYELECTROLYTE MULTILAYERS
• SINGLE
• NANOSTRUCTURES
• DYNAMICS
• CELLS

Plasmon-enhanced fluorescence (PEP) generally requires the samples settled on a metal substrate and the effective enhancement distance is less than 100 nm, which limit its application in intracellular sensing. Herein, we report a nano endoscopy with PEP, effect for sensing analytes inside the extremely small volume samples. The nano endoscopy was fabricated by assembling single nanoporous gold nanowire (PGNW) on the tip of a tungsten needle. It was accurately manipulated to insert into a micro droplet, and an effective sensing was realized at micrometre scale with submicrometer resolution. By taking lysozyme as a model sensing target, a 23 fold improvement of sensitivity was obtained, comparing with that of smooth gold nanowire (SGNW) These results indicated that the nano endoscopy can realize a high spatial resolution sensing, showing its potential application in intracellular sensing.

• 出版日期2017-1-17
• 单位清华大学