Determination of cordycepin content of Cordyceps militaris recombinant rice by high performance liquid chromatography

作者：Wang, Xuesong; Liu, Fang; Li, Fang; Cai, Hongyan; Sun, Wei; Chen, Xuan; Gao, Hong; Shen, Wangyang^*

来源：Tropical Journal of Pharmaceutical Research, 2016, 15(10): 2235-2239.

DOI：10.4314/tjpr.v15i10.23

• Recombinant rice
• Cordycepin
• Chinese caterpillar fungus
• Aweto
• Cordyceps militaris

Purpose: To assess the suitability of high performance liquid chromatography (HPLC) for the determination of cordycepin content of Cordyceps militaris recombinant rice @@@ Methods: Cordyceps militaris recombinant rice was made by mixing brown rice with artificial Chinese caterpillar fungus culture medium powder using twin-screw extrusion technology. Cordycepin content was determined by reversed-phase HPLC with water: acetonitrile (95: 5, v/v) as mobile phase, detection wavelength of 260 nm, and flow rate of 1.0 mL/min. @@@ Results: Cordycepin contents showed good linearity in the range of 1 - 50.0 mu g/mL (r(2) = 0.9996), and while recovery ranged from 103.2 to 109.9 %. Relative standard deviation (RSD), precision and repeatability RSD was 2.38, 0.76 and 1.46 %, respectively. @@@ Conclusion: The HPLC method is simple, fast, accurate and reproducible. It is suitable for determination of cordycepin content of artificial Chinese caterpillar fungus culture medium and brown rice recombinant rice.

• 出版日期2016-10
• 单位武汉轻工大学; 湖北省农业科学院