A modified GEWF solution is cost-saving and effective for lymph node retrieval in resected colorectal carcinoma specimens

作者：Ma, Xiao-Long; Ye, Ju-Xiang; Su, Jing; Qi, Fei-Fei; Meng, Qing-Yang; Shi, Xue-Ying^*

来源：Pathology Research and Practice, 2014, 210(9): 543-547.

DOI：10.1016/j.prp.2014.05.004

• Lymph node revealing solution
• Recycled alcohol
• Eosin
• Colorectal carcinoma
• Staging

Lymph node (LN) retrieval is important for proper staging of colorectal carcinoma. Although various assistant techniques were recommended to facilitate LN identification, most of them were unavoidably time-consuming, resource intensive and costly.
We prepared a modified GEWF solution (RE-GEWF) by use of recycled alcohol and a familiar dye, eosin and investigated its efficacy on 55 colorectal carcinoma specimens. Of the 55 studied cases, 33 of them with <12 LNs (Group A) and 22 with >= 12 LNs were detected (Group B) before RE-GEWF treatment. All were subsequently treated with RE-GEWF for 14-16 h and were inspected again for LNs.
The number of LNs revealed before and after RE-GEWF treatment was 539 and 476 respectively. The mean number of LNs per cases increased from 9.80 /- 6.27 to 18.43 /- 8.77. Twelve accessory LN metastases were found in 9 cases. Upgrade of pN stage was only present in 7 of the Group A cases.
The results show that RE-GEWF is as effective as other reported LN revealing solutions. Use of RE-GEWF not only can assure the quality of LN detection, but also minimize the cost and reduce the release of waste.

• 出版日期2014
• 单位北京大学