Soybean hairy roots produced in vitro by Agrobacterium rhizogenes-mediated transformation

作者：Chen, Li; Cai, Yupeng; Liu, Xiujie; Guo, Chen; Sun, Shi; Wu, Cunxiang; Jiang, Bingjun; Han, Tianfu; Hou, Wensheng^*

来源：Crop Journal, 2018, 6(2): 162-171.

DOI：10.1016/j.cj.2017.08.006

• Agrobacterium rhizogenes
• Hairy root
• Protocol
• Soybean
• Transformation

Soybean is one of the world's most important oil and protein crops. Efficient transformation is a key factor for the improvement of soybean by genetic modification. We describe an optimized protocol for the Agrobacterium rhizogenes-mediated transformation of soybean and the induction of hairy root development in vitro. Cotyledons with 0.5-cm hypocotyls were cut from 5-day-old seedlings and used as explants. After infection and co-cultivation, hairy roots were produced in induction culture medium after 10-12 days. Using this method, 90%-99% of the infected explants of five different cultivars produced hairy roots within one month. Observations using reporter constructs showed that 30%-60% of the hairy roots induced were transformed. Based on high transformation efficiency and short transformation period, this method represents an efficient and rapid platform for study of soybean gene function.

• 出版日期2018-4
• 单位中国农业科学院