PAI-1, CAIX, and VEGFA expressions as prognosis markers in oral squamous cell carcinoma

作者：Peterle Gabriela Tonini; Maia Lucas Lima; Trivilin Leonardo Oliveira; de Oliveira Mayara Mota; dos Santos Joaquim Gasparini; Mendes Suzanny Oliveira; Stur Elaine; Agostini Lidiane Pignaton; Rocha Lilia Alves; Moyses Raquel Ajub; Cury Patricia Maluf; Nunes Fabio Daumas; Louro Iuri Drumond; dos Santos Marcelo; Alvares da Silva Adriana Madeira

来源：Journal of Oral Pathology & Medicine, 2018, 47(6): 566-574.

DOI：10.1111/jop.12721

• CAIX
• hypoxia
• oral squamous cell carcinoma
• PAI-1
• VEGFA

BackgroundIn oral squamous cell carcinoma (OSCC), the HIF-1 complex promotes the expression of genes involved in specific mechanisms of cell survival under hypoxic conditions, such as plasminogen activator inhibitor-1 (PAI-1), carbonic anhydrase 9 (CAIX), and vascular endothelial growth factor A (VEGFA). The study aimed to investigate the presence and prognostic value of PAI-1, CAIX, and VEGFA in OSCC.
Materials and MethodsImmunohistochemistry was used to analyze the expressions of these proteins in 52 tumoral tissue samples of patients with OSCC, surgically treated and followed by a minimum of 24 months after surgery. The correlations between protein expressions and clinicopathological parameters and prognosis were analyzed.
ResultsPositive PAI-1 membrane expression was significantly associated with local disease relapse (P = .027). Multivariate analysis revealed that the positive PAI-1 membrane expression is an independent marker for local disease relapse, with approximately 14-fold increased risk when compared to negative expression (OR = 14.49; CI = 1.40-150.01, P = .025). Strong PAI-1 cytoplasmic expression was significantly associated with the less differentiation grade (P = .027). Strong CAIX membrane expression was significantly associated with local disease-free survival (P = .038). Positive CAIX cytoplasmic expression was significantly associated with lymph node affected (P = .025) and with disease-specific survival (P = .022). Multivariate analysis revealed that the positive CAIX cytoplasmic expression is an independent risk factor for disease-related death, increasing their risk approximately 3-fold when compared to negative expression (HR = 2.84; CI = 1.02-7.87, P = .045). Positive VEGFA cytoplasmic expression was significantly associated with less differentiation grade (P = .035).
ConclusionOur results suggest a potential role for these expressions profiles as tumor prognostic markers in OSCC patients.

• 出版日期2018-7