A Metabonomics Study of Childhood Pneumonia by Rapid Resolution Liquid Chromatography Quadrupole Time-of-flight Mass Spectrometry

作者：Li Jing; Liu Xiao-Lei; Zhao Xu; Li Wei; Dai Yu-Lin^*; Yue Hao^*

来源：Chinese Journal of Analytical Chemistry, 2016, 44(3): 451-455.

DOI：10.11895/j.issn.0253-3820.150872

• Childhood Pneumonia
• Rapid resolution liquid chromatography quadrupole time-of-flight mass spectrometry
• Uric acid
• Metabonomics

In this study, the rapid resolution liquid chromatography quadrupole time-of-flight mass spectrometry (RRLC-QTOF/MS) was used to profile the metabolites of urine samples from Childhood Pneumonia (CP) patients and healthy controls and find the potential biomarkers which can support evidence to early diagnose and cure the disease. Choose 10 CP patients (age 47.72 +/- 2.35 months) and 10 healthy controls (age 46.65 +/- 1.97 months). The urine samples were analyzed by RRLC-QTOF/MS and then the resulting data matrices were analyzed by principal components analysis (PCA) to find the potential biomarkers. Urine samples of CP patients were successfully distinguished from those of healthy controls. A total of two significantly changed metabolites have been found and identified as potential biomarkers. It is suggested that the disorder of purine metabolism and amino acid metabolism may play an important role in the mechanism of CP.

• 出版日期2016-3
• 单位深圳市第三人民医院; 长春中医药大学; 吉林大学