Potential of Capillary Electrophoresis (CE) and Chip-CE with Dual Detection (Capacitively-Coupled Contact less Conductivity Detection ((CD)-D-4) and Fluorescence Detection) for Monitoring of Nicotine and Cotinine Derivatization

作者：Nuchtavorn Nantana; Ryvolova Marketa; Bek Fritz; Macka Mirek; Phechkrajang Chutima; Suntornsuk Leena^*

来源：Analytical Sciences, 2013, 29(3): 339-344.

DOI：10.2116/analsci.29.339

Capillary electrophoresis (CE) coupled with capacitively-coupled contactless conductivity ((CD)-D-4) and fluorescence (FD) detectors and chip-CE for monitoring of nicotine and cotinine derivatization was demonstrated. Separation of the substrates and intermediates could be achieved by CE-(CD)-D-4 in 7 min (R-s %26gt; 2.7) using 45 mM acetic acid (pH 3.0) and this system was applied to detect the intermediate formation. Final fluorescent products could be analyzed by micellar electrokinetic chromatography (MEKC-FD) in 5 mM borate buffer (pH 9.0) containing 10 mM sodium dodecylsulfate (SDS) (%RSD %26lt; 3.00%). Transferring of MEKC-FD to chip-CE allowed for shorter analysis time (2.5 min) and decreased sample consumption. The chip-CE-FD shows good detection and quantitation limits (%26lt; 7.5 mu M) and precision (%RSD %26lt; 2.81%) and was employed to determine nicotine and cotinine in artificial urine. This work reveals the potential of CE and chip-CE with dual detectors as simultaneous, convenient and rapid methods for monitoring pyridine derivatization.

• 出版日期2013-3