A comparison of edge- and basal-plane pyrolytic graphite electrodes towards the sensitive determination of hydrocortisone

作者：Goyal Rajendra N^*; Chatterjee Sanghamitra; Rana Anoop Raj Singh

来源：Talanta, 2010, 83(1): 149-155.

DOI：10.1016/j.talanta.2010.08.054

• Pyrolytic graphite electrode
• Hydrocortisone
• Square-wave voltammetry
• Pharmaceuticals
• Human plasma

Electrochemical sensor employing edge-plane pyrolytic graphite electrode (EPPGE) for the sensitive detection of hydrocortisone (HC) is delineated for the first time The electrochemical properties Ire investigated exercising the cyclic voltammetry and square-wave voltammetry (SWV) When equating with the bare basal-plane pyrolytic graphite electrode (BPPGE) the EPPGE gave better response towards the detection of HC both in terms of sensitivity and detection limit The voltammetric results indicated that EPPGE remarkably enhances the reduction of HC which leads to considerable amelioration of peak current with shift of peak potential to less negative values The difference in the surface morphology of two electrodes has been studied Also the EPPGE delivered an analytical performance for HC with a sensitivity of 45 nA nM(-1) and limit of detection of 88 nM in the concentration range 100-2000 nM The method has been utilized for the determination of HC in pharmaceuticals and real samples The electroanalytical method using EPPGE is the most sensitive method for determination of HC with lowest limit of detection to date The major metabolites present in blood plasma did not intervene with the present investigation as they did no

• 出版日期2010-11-15