Anti-inflammatory effect of grape seed may involve the induction of heme oxygenase-1 and suppression of nuclear factor-kappa B activation

作者：Sung Jeehye; Kim Younghwa; Choi Youngmin; Ham Hyeonmi; Jeong Heon Sang; Lee Junsoo^*

来源：Food Science and Biotechnology, 2011, 20(6): 1713-1719.

DOI：10.1007/s10068-011-0236-x

• grape seed
• anti-inflammation
• nitric oxide
• heme oxygenase-1
• nuclear factor-kappa B

This study aimed to elucidate the antiinflammatory mechanism of grape-seed acetone extract (GSAE) in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. The GSAE at the highest concentration (500 mu g/mL) significantly inhibited nitric oxide production by approximately 78% and suppressed inducible nitric oxide synthase protein expression by approximately 83% compared to LPS-stimulated cells. The GSAE also suppressed inhibitor kappa B alpha phosphorylation and nuclear factor-kappa B (NF-kappa B; p65) transcriptional activity and translocation to the nucleus. In addition, GSAE induced the expression of heme oxygenase-1 (HO-1) in a dose-dependent manner, and that blocking HO-1 activity eliminated the inhibitory effects of the GSAE. The addition of carbon monoxide, a byproduct of heme degradation by HO-1, mimicked the inhibitory action of low concentrations of GSAE. These data suggest that GSAE carries out anti-inflammatory actions in macrophages through the induction of HO-1 and suppression of NF-kappa B activation.

• 出版日期2011-12