科研之友
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摘要
The regulation of proline accumulation in seedlings of rice (oryza sativa L. cv. Badami) was investigated. The increasing concentration of NaCl from 0.5 to 2.5 % progressively increased the proline content in rice. Proline accumulation caused by NaCl was related to protein proteolysis, an increase in OAT, P5CS, P5CR activity, a decrease in PDH activity. The maximum increase in proline content was recorded at 2.5 % NaCl concentration as compared to control and other concentrations of NaCl. The highest significant activity of proline synthesizing enzymes, Delta(1)-Pyrrolline-5-carboxylate synthetase, Delta(1)-Pyrrolline-5-carboxylate reductase and Ornithine-delta-aminotransferase with a lowest activity of proline hydrolysis enzymes; Proline dehydrogenase were also recorded at 2.5 % salinity over control and other concentrations of NaCl with a in-significant increase in the activity of Delta(1)-Pyrrolline-5-carboxylate synthetase and Ornithine-delta-aminotransferase at 0.5 % concentration of NaCl over control. Externally the addition of 300mg MnCl2, 220 ml(-1) (1)/(2) strength Hoagland solution, having 1% NaCl, was seen to increase a 893.9 % in proline content of this variety as compared to control.
- 出版日期2013-7
