A Study of the Interaction Between Malachite Green and Lysozyme by Steady-State Fluorescence

作者：Ding Fei; Liu Wei; Liu Feng; Li Zhi Yuan; Sun Ying^*

来源：Journal of Fluorescence, 2009, 19(5): 783-791.

DOI：10.1007/s10895-009-0475-x

• Malachite green
• Lysozyme
• Fluorescence quenching
• Thermodynamic parameter

The interaction of a N-methylated diaminotriphenylmethane dye, malachite green, with lysozyme was investigated by fluorescence spectroscopic techniques under physiological conditions. The binding parameters have been evaluated by fluorescence quenching methods. The results revealed that malachite green caused the fluorescence quenching of lysozyme through a static quenching procedure. The thermodynamic parameters like Delta H and Delta S were calculated to be -15.33 kJ mol(-1) and 19.47 J mol(-1) K(-1) according to van't Hoff equation, respectively, which proves main interaction between malachite green and lysozyme is hydrophobic forces and hydrogen bond contact. The distance r between donor (lysozyme) and acceptor (malachite green) was obtained to be 3.82 nm according to FO rster's theory. The results of synchronous fluorescence, UV/vis and three-dimensional fluorescence spectra showed that binding of malachite green with lysozyme can induce conformational changes in lysozyme. In addition, the effects of common ions on the constants of lysozyme-malachite green complex were also discussed.

• 出版日期2009-9
• 单位中国农业大学