Unexpected structure of a C. difficile toxin A ligand necessitates an annotation correction in a popular screening library

作者：Zhang Ping; Razi Nahid; Eugenio Luiz; Fentabil Messele; Kitova Elena N; Klassen John S; Bundle David R; Ng Kenneth K S^*; Ling Chang Chun

来源：Chemical Communications, 2011, 47(45): 12397-12399.

DOI：10.1039/c1cc15344g

The structure of the pentasaccharide S259-1 in the Consortium for Functional Glycomics was investigated using a variety of techniques. Surprisingly, the structure differs from the structure assumed from the previously established specificity of the human fucosyltransferase FUT-III used in the last step of chemoenzymatic synthesis. When presented with a tetrasaccharide substrate containing both type I and type II disaccharide moieties, the enzyme generates a pentasaccharide in which the type II moiety is preferentially fucosylated. The unexpected product generated by FUT-III in this case highlights the importance of performing detailed structural analysis on products generated by enzymes.

• 出版日期2011