Identification of a sugar beet BvM14-MADS box gene through differential gene expression analysis of monosomic addition line M14

作者：Ma Chunquan; Wang Yuguang; Wang Yuting; Wang Lifa; Chen Sixue; Li Haiying^*

来源：Journal of Plant Physiology, 2011, 168(16): 1980-1986.

DOI：10.1016/j.jplph.2011.05.027

• BvM14-MADS box
• Differential expression
• Sugar beet
• M14
• Diplospory

Monosomic addition line M14 carrying an additional chromosome 9 from Beta corolliflora Zosimovic ex Buttler was obtained through hybridization between the wild species B. corolliflora and a cultivated species Beta vulgaris L var Saccharifera Alef. The M14 line showed diplosporic reproduction and stress tolerance. To identify differentially expressed genes in M14, a subtractive cDNA library was prepared by suppression subtractive hybridization (SSH) between M14 (2n =18 + 1) and B. vulgaris (2n = 18). A total of 190 unique sequences were identified in the library and their putative functions were analyzed using Gene Ontology (GO). One of the genes, designated as BvM14-MADS box, encodes a MADS box transcription factor. It was cloned from M14 and over-expressed in transgenic tobacco plants. Interestingly, this gene was located on chromosome 2 of B. vulgaris, not on the additional chromosome 9. Overexpression of BvM14-MADS box led to significant phenotypic changes in tobacco. The differential expression of BvM14-MADS box gene in M14 may be caused by the interaction between the additional chromosome 9 from B. corolliflora and the B. vulgaris chromosomes in M14.

• 出版日期2011-11-1
• 单位黑龙江大学