A YEAST GENE THAT IS ESSENTIAL FOR RELEASE FROM GLUCOSE REPRESSION ENCODES A PROTEIN-KINASE

作者：CELENZA JL^*; CARLSON M

来源：Science, 1986, 233(4769): 1175-1180.

DOI：10.1126/science.3526554

<jats:p>
The
<jats:italic>SNF1</jats:italic>
gene plays a central role in carbon catabolite repression in the yeast
<jats:italic>Saccharomyces cerevisiae</jats:italic>
, namely that
<jats:italic>SNF1</jats:italic>
function is required for expression of glucose-repressible genes. The nucleotide sequence of the cloned
<jats:italic>SNF1</jats:italic>
gene was determined, and the predicted amino acid sequence shows that
<jats:italic>SNF1</jats:italic>
encodes a 72,040-dalton polypeptide that has significant homology to the conserved catalytic domain of mammalian protein kinases. Specific antisera were prepared and used to identify the
<jats:italic>SNF1</jats:italic>
protein. The protein was shown to transfer phosphate from adenosine triphosphate to serine and threonine residues in an in vitro autophosphorylation reaction. These findings indicate that
<jats:italic>SNF1</jats:italic>
encodes a protein kinase and suggest that protein phosphorylation plays a critical role in regulation by carbon catabolite repression in eukaryotic cells.
</jats:p>

• 出版日期1986-9-12