Agitation-induced aggregation and subvisible particulate formation in model proteins

作者：Jayaraman Murali; Buck Patrick M; Ignatius Arun Alphonse; King Kevin R; Wang Wei^*

来源：European Journal of Pharmaceutics and Biopharmaceutics, 2014, 87(2): 299-309.

DOI：10.1016/j.ejpb.2014.01.004

• Protein
• Agitation
• Particle
• Aggregation
• Stability
• Unfolding

The kinetics of agitation-induced subvisible particle formation was investigated for a few model proteins - human serum albumin (HSA), hen egg white lysozyme (HEWL), and a monoclonal antibody (IgG2). Experiments were carried out for the first time under relatively low protein concentration and low agitation speed to investigate the details of subvisible particle formation at the initial phase of aggregation (<2%) process. Upon agitation, both soluble higher molecular mass species (HMMS) and subvisible particles (SbVPs) formed at different rates, and via different mechanisms. Agitation enhanced exposure of hydrophobic sites in HSA but did not cause detectable structural changes in HEWL and IgG2. SbVPs from HSA partially dissociates in a neutral pH buffer (SEC mobile phase) but does not upon dilution in the same formulation buffer. Opposite results were obtained for SbVPs from IgG2 and HEWL. Neither the relative hydrophobic surface area nor the Tm of the model proteins seems to be an indicator of tendency for agitation-mediated SbVP formation. Taken together, our data suggests that agitation-induced SbVP formation can occur through different mechanisms and can vary, depending on the protein and solution conditions.

• 出版日期2014-7