Australian bat lyssavirus infection in two horses

作者：Shinwari Mustaghfira Wafa; Annand Edward J; Driver Luke; Warrilow David; Harrower Bruce; Allcock Richard J N; Pukallus Dennis; Harper Jennifer; Bingham John; Kung Nina; Diallo Ibrahim S^*

来源：Veterinary Microbiology, 2014, 173(3-4): 224-231.

DOI：10.1016/j.vetmic.2014.07.029

• Australian bat lyssavirus
• Equine non-suppurative meningoencephalomyelitis
• Negri bodies
• Yellow-bellied sheathtail bat

In May 2013, the first cases of Australian bat lyssavirus infections in domestic animals were identified in Australia. Two horses (filly-H1 and gelding-H2) were infected with the Yellow-bellied sheathtail bat (YBST) variant of Australian bat lyssavirus (ABLV). The horses presented with neurological signs, pyrexia and progressing ataxia. Intra-cytoplasmic inclusion bodies (Negri bodies) were detected in some Purkinje neurons in haematoxylin and eosin (H%26E) stained sections from the brain of one of the two infected horses (H2) by histological examination. A morphological diagnosis of sub-acute moderate non-suppurative, predominantly angiocentric, meningo-encephalomyelitis of viral aetiology was made. The presumptive diagnosis of ABLV infection was confirmed by the positive testing of the affected brain tissue from (H2) in a range of laboratory tests including fluorescent antibody test (FAT) and real-time PCR targeting the nucleocapsid (N) gene. Retrospective testing of the oral swab from (H1) in the real-time PCR also returned a positive result. The FAT and immunohistochemistry (IHC) revealed an abundance of ABLV antigen throughout the examined brain sections. ABLV was isolated from the brain (H2) and oral swab/saliva (H1) in the neuroblastoma cell line (MNA). Alignment of the genome sequence revealed a 97.7% identity with the YBST ABLV strain.

• 出版日期2014-10-10
• 单位CSIRO