Evaluation of Lama glama semen viscosity with a cone-plate rotational viscometer

作者：Casaretto C^*; Martinez Sarrasague M; Giuliano S; Rubin de Celis E; Gambarotta M; Carretero I; Miragaya M

来源：Andrologia, 2012, 44: 335-341.

DOI：10.1111/j.1439-0272.2011.01186.x

• Lama
• rheology
• semen
• viscosity

Llama semen is highly viscous. This characteristic is usually evaluated subjectively by measuring the thread formed when carefully pippeting a sample of semen. The aims of this study were (i) to objectively determine and analyse llama semen viscosity, (ii) to compare semen viscosity between ejaculates of the same male as well as between different males, (iii) to study the correlation between viscosity and other semen characteristics and (iv) to evaluate the effect of collagenase on semen viscosity. Semen viscosity was evaluated using a cone-plate Brookfield rotational viscometer. A non Newtonian, pseudoplastic behaviour was observed in the 45 semen samples evaluated. Rheological parameters were determined obtaining the following results (mean +/- SD): apparent viscosity at 11.5 s-1: 46.71 +/- 26.8 cpoise and at 115 s-1: 12.61 +/- 4.1 cpoise; structural viscosity (K) (dyne s cm-2): 2.18 +/- 1.4 and coefficient of consistency (n): 0.45 +/- 0.1. Statistical differences were found between different ejaculates of the same male for structural viscosity and apparent viscosity at 11.5 s-1 (P %26lt; 0.01). Correlation was found only between coefficient of consistency (n) and sperm concentration (P %26lt; 0.01). Significant differences for coefficient of consistency (n) and viscosity at 115 s-1 were found between samples incubated with and without collagenase (P %26lt; 0.05).

• 出版日期2012-5