A search for novel markers of alcohol abuse using magnetic beads and MALDI-TOF/TOF mass spectrometry

作者：Sogawa Kazuyuki; Satoh Mamoru; Kodera Yoshio; Tomonaga Takeshi; Iyo Masaomi; Nomura Fumio^*

来源：Proteomics - Clinical Applications, 2009, 3(7): 821-828.

DOI：10.1002/prca.200800174

• Alcoholism
• MALDI
• SELDI
• Serum

Objective markers are required to assess excessive alcohol consumption, which can lead to a various medical and social problems. In this study, we carried out serum peptidome analyses using the ClinProt (TM) system, which consists of magnetic beads and MALDI-TOF/TOF MS, to find novel biomarkers of alcohol abuse in 16 chronic alcoholic patients that were hospitalized for a rehabilitation program. A total of 22 peaks were found to be significantly altered during abstinence. Out of these 22 peaks, 3 peaks that had an m/z of 3000 or less and substantial peak intensities were subjected to MS/MS analysis followed by a MASCOT search. The 1466 Da and the 1616 Da peptides were upregulated. on admission and were identified as fragments of fibrinopeptide A and phosphorylated fibrinopeptide A, respectively. On the other hand, the 2660 Da peptide, which was downregulated on admission and increased during abstinence, was identified as a fragment of the fibrinogen alpha C chain. These peaks were not detectable by the SELDI-TOF MS ProteinChip (R) system analysis. The alterations in these peaks induced by alcohol abuse were also seen in gamma glutamyltransferase nonresponders. These protein fragments may be additional biomarkers for excessive alcohol drinking.

• 出版日期2009-7