A negative-pressure-driven microfluidic chip for the rapid detection of a bladder cancer biomarker in urine using bead-based enzyme-linked immunosorbent assay

作者：Lin Yen Heng^*; Chen Ying Ju; Lai Chao Sung; Chen Yi Ting; Chen Chien Lun; Yu Jau Song; Chang Yu Sun

来源：Biomicrofluidics, 2013, 7(2): 024103.

DOI：10.1063/1.4794974

• biochemistry
• biological organs
• biomedical equipment
• bioMEMS
• cancer
• microfluidics
• microvalves
• molecular biophysics
• patient diagnosis
• proteins

This paper describes an integrated microfluidic chip that is capable of rapidly and quantitatively measuring the concentration of a bladder cancer biomarker, apolipoprotein A1, in urine samples. All of the microfluidic components, including the fluid transport system, the micro-valve, and the micro-mixer, were driven by negative pressure, which simplifies the use of the chip and facilitates commercialization. Magnetic beads were used as a solid support for the primary antibody, which captured apolipoprotein A1 in patients%26apos; urine. Because of the three-dimensional structure of the magnetic beads, the concentration range of the target that could be detected was as high as 2000 ng ml-1. Because this concentration is 100 times higher than that quantifiable using a 96-well plate with the same enzyme-linked immunosorbent assay (ELISA) kit, the dilution of the patient%26apos;s urine can be avoided or greatly reduced. The limit of detection was determined to be approximately 10 ng ml-1, which is lower than the cutoff value for diagnosing bladder cancer (11.16 ng ml-1). When the values measured using the microfluidic chip were compared with those measured using conventional ELISA using a 96-well plate for five patients, the deviations were 0.9%, 6.8%, 9.4%, 1.8%, and 5.8%. The entire measurement time is 6-fold faster than that of conventional ELISA. This microfluidic device shows significant potential for point-of-care applications.

• 出版日期2013-3
• 单位长春大学; 河北医科大学