Molecularly imprinted submicronspheres for applications in a novel model biosensor-film

作者：Morelli Ilaria; Chiono Valeria; Vozzi Giovanni^*; Ciardelli Gianluca; Silvestri Davide; Giusti Paolo

来源：Sensors and Actuators B: Chemical , 2010, 150(1): 394-401.

DOI：10.1016/j.snb.2010.06.046

• Biosensor
• Molecular imprinted polymers
• Submicronspheres
• Methacrylic acid
• Pentaerythritol triacrylate
• Phenylalanine
• Trimethylolpropane trimethacrylate

Two different molecularly imprinted polymers (MIP1 and MIP2) in the form of submicron-particles were obtained by radical polymerization, using acetonitrile as a solvent, methacrylic acid (MAA) as a functional monomer, a cross-linking agent (trimethylolpropane trimethacrylate (TRIM) for MIP1 and pentaerythritol triacrylate (PETRA) for MIP2) and phenylalanine aminoacid (Phe) as a template molecule The extraction of Phe template from polymer submicron-particles by washing steps allowed the formation of flee recognition sites for the selective rebinding of template molecule Rebinding ability was evaluated in acetonitrile and in phosphate buffered saline by chromatographic methods and compared to that of corresponding control polymers As MIP1 showed a superior specificity towards Phe rebinding as compared to MIP2, it was selected as a component of a poly(L-lactic acid) (PLLA) based model sensor-scaffold Impedance measurements, carried out on PLLA films loaded with as-produced, extracted or rebound MIP1 submicron-particles demonstrated the possibility to build a scaffold capable of sensing the amount of rebound template In the future, devices incorporating MIP submicron-particles able to rebind extracell

• 出版日期2010-9-21