Automated nutrient screening system enables high-throughput optimisation of microalgae production conditions

作者：Radzun Khairul Adzfa; Wolf Juliane; Jakob Gisela; Zhang Eugene; Stephens Evan; Ross Ian; Hankamer Ben^*

来源：Biotechnology for Biofuels, 2015, 8(1): UNSP 65.

DOI：10.1186/s13068-015-0238-7

• Algae
• Nutrient
• Biomass
• Screening
• High-throughput
• Growth

Background: Microalgae provide an excellent platform for the production of high-value-products and are increasingly being recognised as a promising production system for biomass, animal feeds and renewable fuels.
Results: Here, we describe an automated screen, to enable high-throughput optimisation of 12 nutrients for microalgae production. Its miniaturised 1,728 multiwell format allows multiple microalgae strains to be simultaneously screened using a two-step process. Step 1 optimises the primary elements nitrogen and phosphorous. Step 2 uses Box-Behnken analysis to define the highest growth rates within the large multidimensional space tested (Ca, Mg, Fe, Mn, Zn, Cu, B, Se, V, Si) at three levels (-1, 0, 1). The highest specific growth rates and maximum OD750 values provide a measure for continuous and batch culture.
Conclusion: The screen identified the main nutrient effects on growth, pairwise nutrient interactions (for example, Ca-Mg) and the best production conditions of the sampled statistical space providing the basis for a targeted full factorial screen to assist with optimisation of algae production.

• 出版日期2015-4-11