Development of an oral mucosa equivalent using a porcine dermal matrix

作者：Basso F G^*; Hebling J; Marcelo C L; Costa C A de Souza; Feinberg S E

来源：British Journal of Oral and Maxillofacial Surgery, 2017, 55(3): 308-311.

DOI：10.1016/j.bjoms.2016.09.019

• Oral keratinocytes
• organotypic cell culture
• oral mucosa

We evaluated the suitability of a porcine acellular dermal matrix for the development of a 3-dimensional oral mucosal equivalent using an exvivo-produced oral mucosal equivalent (EVPOME). Oral keratinocytes were seeded in a submerged model, and then in an air-liquid interphase model, using Transwell((R)) inserts. EVPOME showed good cell viability and increased glucose consumption over time. Histological evaluation showed that stratified differentiated epithelium had formed in all matrices.

• 出版日期2017-4
• 单位Univ Estadual Paulista