A gene-delivery system specific for hepatoma cells and an intracellular kinase signal based on human liver-specific bionanocapsules and signal-responsive artificial polymer

作者：Oishi Jun; Jung Joohee; Tsuchiya Akira; Toita Riki; Kang Jeong Hun; Mori Takeshi; Niidome Takuro; Tanizawa Katsuyuki; Kuroda Shun'ichi; Katayama Yoshiki^*

来源：International Journal of Pharmaceutics, 2010, 396(1-2): 174-178.

DOI：10.1016/j.ijpharm.2010.06.012

• Gene therapy
• Intracellular signal
• Protein kinase
• Tumor-targeted gene delivery
• Hepatocellular carcinoma

Recently, our group has proposed a novel gene-regulation system responding to CAMP-dependent protein kinase (PKA) that has been applied to living cells. In this study, human liver-specific bionanocapsules (BNCs) are used as a gene-delivery system to increase transfection efficiency and to target specific cell types BNCs can efficiently deliver a target gene to human hepatocytes and hepatoma cells in vitro or in vivo The combination of a signal-responsive gene-delivery system with BNCs led to an increase in the transfection efficiency and selectivity for hepatoma cells. Expression from the delivered gene was identified from PICA-activated hepatoma cells (HepG2), but not from colon tumor cells (MIX) These results show t

• 出版日期2010-8-30