A Molecular Method to Detect Wound Cells in Bloodstains Resultant of Sharp Force Injuries for Crime Scene Reconstruction

作者：Johnson Donald J^*; Raymond David E; Chen Cynthia; Quon Matthew; Lis Julian; Choi Mo Re; Lopez Christopher; Han Aileen; de Leon Ray D; Bir Cynthia

来源：Journal of Forensic Sciences, 2018, 63(3): 842-848.

DOI：10.1111/1556-4029.13623

• forensic science
• criminalistics
• forensic biology
• bloodstain patterns
• sharp force injuries
• microRNA
• crime scene reconstruction

Previous research by the authors on an animal model showed that bloodstains can contain additional information about their somatic origin in the form of wound cells. Bloodstains produced by a gunshot wound to the head were distinguished from bloodstains produced by a gunshot wound to the chest by testing the stains for a brain microRNA marker. In this study, the effectiveness of the technique was examined on blood drops shed externally from a stab wound to the liver of rat carcasses. Specifically, investigations were conducted on the liver microRNA marker, rno-mir-122-3p, with the QIAGEN miScript System, and PCR analysis. Between the two stabbing methods used, 67% of the scalpel blades and 57% of the blood drops tested positive for rno-mir-122-3p; however, other samples tested negative giving inconclusive results as to the wound-of-origin. The amount of the liver cells in the bloodstains appeared to be related to the extent of trauma.

• 出版日期2018-5