Degradation of amyloid peptide by neprilysin expressed from Borna disease virus vector

作者：Sakai Madoka; Ueda Sakiho; Daito Takuji; Asada Utsugi Megumi; Komatsu Yumiko; Kinoshita Ayae; Maki Takakuni; Kuzuya Akira; Takahashi Ryosuke; Makino Akiko^*; Tomonaga Keizo

来源：Microbiology and Immunology, 2018, 62(7): 467-472.

DOI：10.1111/1348-0421.12602

• Alzheimer disease
• amyloid
• Borna disease virus vector
• neprilysin

Accumulation of amyloid (A40 and A42) in the brain is a characteristic of Alzheimer disease (AD). Because neprilysin (NEP) is a major A-degrading enzyme, NEP delivery in the brain is a promising gene therapy for AD. Borna disease virus (BoDV) vector enables long-term transduction of foreign genes in the central nerve system. Here, the proteolytic ability of NEP transduced by the BoDV vector was evaluated and it was found that the amounts of A40 and A42 decreased significantly, suggesting that NEP expressed from the BoDV vector is functional in that it degrades A.

• 出版日期2018-7