An integrative genomics screen uncovers ncRNA T-UCR functions in neuroblastoma tumours

作者：Mestdagh P; Fredlund E; Pattyn F; Rihani A; Van Maerken T; Vermeulen J; Kumps C; Menten B; De Preter K; Schramm A; Schulte J; Noguera R; Schleiermacher G; Janoueix Lerosey I; Laureys G; Powel R; Nittner D; Marine J C; Ringner M; Speleman F; Vandesompele J^*

来源：Oncogene, 2010, 29(24): 3583-3592.

DOI：10.1038/onc.2010.106

• neuroblastoma
• T-UCR
• non-coding RNA

Different classes of non-coding RNAs, including microRNAs, have recently been implicated in the process of tumourigenesis. In this study, we examined the expression and putative functions of a novel class of non-coding RNAs known as transcribed ultraconserved regions (T-UCRs) in neuroblastoma. Genome-wide expression pro. ling revealed correlations between specific T-UCR expression levels and important clinicogenetic parameters such as MYCN amplification status. A functional genomics approach based on the integration of multi-level transcriptome data was adapted to gain insights into T-UCR functions. Assignments of T-UCRs to cellular processes such as TP53 response, differentiation and proliferation were verified using various cellular model systems. For the first time, our results de. ne a T-UCR expression landscape in neuroblastoma and suggest widespread T-UCR involvement in diverse cellular processes that are deregulated in the process of tumourigenesis. Oncogene (2010) 29, 3583-3592; doi:10.1038/onc.2010.106; published online 12 April 2010

• 出版日期2010-6-17