AUTOPHAGY SIGNALLING IS DIFFERENTIALLY MODULATED BY miR-204 IN CONTEXT OF INNATE IMMUNITY INDUCTION

作者：Hayrabedyan Soren^*; Mincheff Milcho; Zasheva Diana; Manolova Nelly; Todorova Krassimira

来源：Comptes Rendus de l Academie Bulgare des Sciences, 2013, 66(1): 127-132.

DOI：10.7546/cr-2013-66-1-13101331-16

• prostate cancer
• ATG16L
• LC3
• micro-RNA
• ie-DAP

This study investigates the initiation autophagy complex factor ATG16L and the most common mature autophagosomes marker - LC3 expression levels in conditions of innate immunity challenge and the modulatory role of miR-204 on it. The effect of miR-204 mimic transfection on Gram-synthetic bacterial peptidoglycan challenged human prostate cancer cell lines LNCaP (p53+/+, AR+), and PC3 (p53-/-, AR-) was evaluated using flow cytometric (FCS) and immunofluorescent detection of the expression of ATG16L and LC3. ATG16L and LC3 have an increased fluorescence observation in LNCaP cells treated with ie-DAP for 6 h, while in PC3 cells rather the opposite effect is observed. Flow cytometry demonstrated that ie-DAP and miR-204 have strongest effect on LC3 and lesser prominent one on ATG16L, and no effect when only ie-DAP is applied in LNCaP cells. In PC3 cells, conversely, they exert a rather negative effect with strongest impact of the concomitant application of miR-204 and ie-DAP on LC3 and no effect on ATG16L. Our data suggest that miR-204 differentially impacts autophagy signalling in prostate cancer cells in concordance with the p53 status, raising possibilities for targeted therapy augmentation through autophagy modulation using artificial micro-RNA.

• 出版日期2013