Deep phenotyping of Tregs identifies an immune signature for idiopathic aplastic anemia and predicts response to treatment

作者：Kordasti Shahram; Costantini Benedetta; Seidl Thomas; Abellan Pilar Perez; Llordella Marc Martinez; McLornan Donal; Diggins Kirsten E; Kulasekararaj Austin; Benfatto Cinzia; Feng Xingmin; Smith Alexander; Mian Syed A; Melchiotti Rossella; de Rinaldis Emanuele; Ellis Richard; Petrov Nedyalko; Povoleri Giovanni A M; Chung Sun Sook; Thomas N Shaun B; Farzaneh Farzin; Irish Jonathan M; Heck Susanne; Young Neal S; Marsh Judith C W; Mufti Ghulam J

来源：Blood, 2016, 128(9): 1193-1205.

DOI：10.1182/blood-2016-03-703702

Idiopathic aplastic anemia (AA) is an immune-mediated and serious form of bone marrow failure. Akin to other autoimmune diseases, we have previously shown that in AA regulatory T cells (Tregs) are reduced in numberand function. The aim of this study was to further characterize Treg subpopulations in AA and investigate the potential correlation between specific Treg subsets and response to immunosuppressive therapy (IST) as well as their in vitro expandability for potential clinical use. Using mass cytometry and an unbiased multidimensional analytical approach, we identified 2 specific human Treg subpopulations (Treg A and Treg B) with distinct phenotypes, gene expression, expandability, and function. Treg B predominates in IST responder patients, has a memory/activated phenotype (with higher expression of CD95, CCR4, andCD45ROwithin FOXP3(hi), CD127(lo) Tregs), expresses the interleukin-2 (IL-2)/STAT5 pathway and cell-cycle commitment genes. Furthermore, in vitro-expanded Tregs become functional and take on the characteristics of Treg B. Collectively, this study identifies human Treg subpopulations that can be used as predictive biomarkers for response to IST in AA and potentially other autoimmune diseases. We also show that Tregs from AA patients are IL-2-sensitive and expandable in vitro, suggesting novel therapeutic approaches such as low-dose IL-2 therapy and/or expanded autologous Tregs and meriting further exploration.

• 出版日期2016-9-1