Detection of Listeria monocytogenes in pork and beef using the VIDAS (R) LMO2 automated enzyme linked immunoassay method

作者：Meyer Cornelia^*; Fredriksson Ahomaa Maria; Sperner Brigitte; Maertlbauer Erwin

来源：Meat Science, 2011, 88(3): 594-596.

DOI：10.1016/j.meatsci.2011.01.035

• Listeria spp.
• Listeria monocytogenes
• VIDAS
• Raw meat

Listeria (L) monocytogenes, a foodborne pathogen, is known to be a possible contaminant of foods during production and processing. Samples (n = 985) of raw meat and by-products obtained from beef and pork were first screened by the VIDAS system for the presence of Listeria spp., followed by testing for the presence of L monocytogenes. Positive L monocytogenes results were confirmed by plating on selective agars: 14% of the samples were positive for Listeria and 4% tested positive for L monocytogenes, of which 3% were confirmed on selective agars. In by-products (17%) the contamination with listeriae was higher than in meat cuts (10%). Only samples strongly positive for Listeria spp. by VIDAS were positive for L monocytogenes. Overall, the prevalence of L. monocytogenes in beef and pork samples was rather low in comparison to most previous studies. The VIDAS system was shown to be a suitable method for screening out Listeria-negative samples; the main advantage being a markedly reduced assay time.

• 出版日期2011-7